Researchers at the Max Planck Institute for Marine Microbiology in Bremen, Germany, have now overturned that long-standing assumption.
The findings reveal a previously overlooked way in which microbial carbon can enter animal food webs.
Laboratory experiments confirmed that enzymes from phylogenetically distant animals –including a sponge, an earthworm and a springtail – also degrade microbial PHAs.
More fundamentally, they reveal that animals can exploit a microbial carbon reserve that had previously been thought to be inaccessible to them.
“Animals have probably been feeding on nature’s original bioplastic for hundreds of millions of years – we’re only discovering it now.”
Long before humans discovered biodegradable plastics, microorganisms had already invented their own. Many bacteria and archaea produce natural bioplastics called polyhydroxyalkanoates (PHAs), storing them inside their cells as reserves of carbon and energy.
Until now, scientists thought that only microorganisms themselves could break down these substances. Researchers at the Max Planck Institute for Marine Microbiology in Bremen, Germany, have now overturned that long-standing assumption. In a study published in Nature Ecology & Evolution, they show that animals ranging from marine worms and starfish to terrestrial species including earthworms have enzymes capable of degrading microbial PHAs. The findings reveal a previously overlooked way in which microbial carbon can enter animal food webs.
The story began with an unusual marine worm called Olavius algarvensis. It has neither a mouth nor a gut. Instead, it farms symbiotic bacteria beneath its skin and digests them for food.“One of the worm’s bacterial symbionts stores enormous amounts of carbon as PHA,” says corresponding author Nicole Dubilier, Director at the Max Planck Institute for Marine Microbiology. “We wondered whether the worm had evolved a way to access this rich energy reserve.”
The answer was: Yes. The researchers discovered an enzyme in the worm that breaks down microbial PHAs into small molecules animals can use. High resolution images further showed that the enzyme is produced exactly where the worm digests its symbionts. This suggests that the worm can access the PHA stored by its bacterial partners.
The team then searched genomes across the animal kingdom and found related enzymes surprisingly often – in more than 66 animal species representing nine different phyla. Laboratory experiments confirmed that enzymes from phylogenetically distant animals –including a sponge, an earthworm and a springtail – also degrade microbial PHAs. “This was the real surprise,” says first author Caroline Zeidler from the Max Planck Institute for Marine Microbiology. “What started as a discovery in a single marine worm turned out to be a widespread capability shared by animals from very different branches of the tree of life.”
Microbial PHAs occur naturally in soils, sediments and aquatic environments worldwide. They are produced whenever microorganisms store excess carbon for later use and are among the few naturally occurring plastics that are completely biodegradable. Because PHAs are increasingly manufactured as sustainable alternatives to conventional plastics, understanding how they are degraded in nature has become an important area of research.
The new findings by Dubilier and her team suggest that animals, together with microorganisms, may contribute to the breakdown of these natural bioplastics. More fundamentally, they reveal that animals can exploit a microbial carbon reserve that had previously been thought to be inaccessible to them.
“Our study changes our understanding of who can use these microbial carbon stores,” says co-corresponding author Maggie Sogin, who carried out much of the work at the Max Planck Institute for Marine Microbiology and is now Assistant Professor at the University of California, Merced. “Animals have probably been feeding on nature’s original bioplastic for hundreds of millions of years – we’re only discovering it now.”
